FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209785
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.15015372881759897No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.13156326715446767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC605.604852E-849.6004453
GTAACCG409.307998E-442.49449
CTGTGCG505.4409138E-542.49449
TGGTATC1800.040.152742
TAGTACT655.527063E-639.244314
GTCGAGT450.001661023437.77288
GTGGTAT1850.036.7694441
GGTATCA3550.035.9278871
GTATCAA6550.035.699341
TAGGTCG301.5928067E-435.4204420-21
GTCGAGG1650.033.4804348
CTAGGAT652.5411253E-432.703593
TATCAAC7950.032.6213152
AGTCGAG2000.031.8783977
CCTAGTA703.924416E-430.3676192
CGTCTAC353.939305E-430.35675854-55
GCTCGAT353.939305E-430.35675864-65
CAACGCA8600.028.672685
ATCAACG8600.028.672683
GCCTAGT755.877545E-428.3431111
Produced by FastQC (version 0.11.5)