FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.8966416693837626No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.13042060645582002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA357.6959615E-539.2857131
CGCAGAG350.003790905731.4285728
GCAGAGT506.2574656E-427.59
AACGCAG400.007284605427.4999986
ACGCAGA400.007284605427.4999987
TATCAAC400.007284605427.4999982
CAACGCA400.007284605427.4999985
ACACATC855.699795E-419.41176455
TACACAT950.001205015717.36842254
ATACACA1000.001699922616.553
TATACAC1050.002356440315.71428652
TTATACA1300.00975121212.69230751
Produced by FastQC (version 0.11.5)