FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334068
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11390.3409485493971287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3800.039.0837362
GTGGTAT3850.038.5992771
GTCCTCG350.00408106131.4512631
TTCGAAG350.00409305231.432422
AATACGG350.00410206331.4183045
CCTTAAT507.016375E-427.5033722
CGAGGGT507.016375E-427.50337210
GTATCAA13400.026.9037421
TATAGTA550.001223857625.0030612
TATCAAC14650.024.0302472
AGCGCGA701.7972325E-423.57078635
GGTATCA6800.023.0473923
ATATAGT600.002024009822.9332141
GCGCGAG600.002032846222.91604436
AGAGTAC9900.022.22494511
CAACGCA15550.021.9220985
CAGAGTA10000.021.72766110
TCAACGC16050.021.4104524
ATCAACG16200.021.3851053
GTATAAA650.003221483421.1691191
Produced by FastQC (version 0.11.5)