FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27267
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.2933949462720505No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.28972750944364983No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.2493857043312429No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.2163787728756372No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.20170902556203468No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.17970440459163092No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.1650346572780284No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11002310485201892No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10268823119521765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA150.001018734384.999995
TCAGATG150.001018734384.999997
AGCGTCA150.001018734384.999993
TGGTATC455.5570126E-966.111112
GTGGTAT455.5570126E-966.111111
CCTCTCC200.003189801763.758
GCGTCAG200.003189801763.754
ATATCAA401.8862556E-763.751
TCGACGT250.007715304451.09
GTCGACG250.007715304451.08
AGATGTG250.007715304451.09
ACCTCCA250.007715304451.07
TATCAAC2850.046.2280732
GTATCAA2350.045.212771
ATCAACG3300.039.924243
TCAACGC3450.038.1884044
CAACGCA3500.037.6428575
AACGCAG3650.036.0958946
CTTCTGA500.002741707734.01
CGTTTTT250.002765853734.012-13
Produced by FastQC (version 0.11.5)