FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27267
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2761.012212564638574No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.4327575457512744No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.2823926357868486No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.23838339384604101No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.18337184142003154No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGG150.005718770455.0110337
TGGTATC406.8961526E-848.046332
GTGGTAT451.7163438E-742.7863541
TTTAAGG300.001890938336.6740243
GTATCAA2350.033.9429741
TCCTCAG350.00402633531.43487411
TATCAAC2600.030.6229342
GTCGAGG1350.030.5616848
CGAGGTT1300.029.62132310
TCGAGGT1300.029.6213239
GAGGTTT1350.028.52423911
CGGGGGA506.8477757E-427.50551617
AGAGTAC1900.027.50551411
CAGAGTA1950.026.80024510
AGGTTTT1450.026.55704912
ATCAACG2850.026.0100443
GGTATCA858.262359E-725.8875451
GGTTTTT1500.025.67181613
TACGGGG1407.2759576E-1225.54083415
ACGGGGA651.0497701E-425.38970616
Produced by FastQC (version 0.11.5)