FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793932
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.13716539955562945No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10660.13426842601129568No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.12293244257694613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6750.037.159251
GTATCAA23550.036.284721
ACCGATC601.5958295E-435.4138578
TATCAAC27400.033.0419462
CAACGCA28150.032.3126035
ATCAACG28000.032.1821023
TCAACGC28700.031.545274
AACGCAG29800.030.951386
TGGTATC8550.030.8220922
AGTCGAG6200.029.4734697
TATTGCG600.006806968728.3310879
CGCAGAG33250.027.4790238
GTACTAG1554.434696E-927.4275461
ACTACGT957.4415046E-526.8450496
TACTACG957.4415046E-526.8450495
ACGTGCG957.451081E-526.8399759
ACGCAGA34600.026.5296887
GTCGAGG5350.026.2128758
AGCGTCA2650.025.6631933
TCGAGGT5300.025.6583429
Produced by FastQC (version 0.11.5)