FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84427
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.30085162329586507No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT1260.1492413564381063No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.13265898350053892No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.10304760325488291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA750.073.6666641
AATGCTT200.0032131463.754
TTTAAGC250.007771393751.0000043
GTTCACA250.007771393751.0000041
CTTTAAG250.007771393751.0000042
TATACTA250.007771393751.0000045
CACCCTC250.007771393751.0000042
CTTGCGG354.8014388E-448.571433
TATCAAC5550.045.180182
GTATCAA4550.042.9670331
AACGCAG5750.042.1304326
ATCAACG6050.041.446283
TCAACGC6200.040.4435464
CAACGCA6300.039.8015865
ACGCAGA6450.037.558147
CGCAGAG6700.036.1567158
GCAGAGT6700.035.522399
GTGGTAT1202.8012437E-1035.4166641
ATGCTTG500.00277444134.0000045
TGGTATC1306.6029315E-1032.692312
Produced by FastQC (version 0.11.5)