FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201200
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.18687872763419483No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.12326043737574553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC354.8189668E-448.5683027
CGCTTGG452.9191415E-547.2191858
GCGTCAG551.7566381E-646.3606574
CCGCTTG409.3040004E-442.497277
AGCGTCA603.197034E-642.4972653
GTATCAA10950.038.819941
AACGCAG12300.038.3511926
TATCAAC12250.037.120062
ATCAACG12850.035.717553
CAACGCA12950.035.113575
TCAACGC13100.034.7115064
CGCAGAA250.002784326734.00626430-31
CATTTAG500.002784425933.9978142
ACGCAGA13700.033.5014957
CGCAGAG13950.032.9011088
ATATCAA1201.1114025E-831.8808751
GCCGCTT550.00444077630.9071036
GCAGAGT15000.030.598039
CGTCAGA853.4782253E-529.9980685
CGTCGCG300.00679841528.33503314-15
Produced by FastQC (version 0.11.5)