FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201200
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4500.22365805168986083No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC453.7769065E-430.5660553
CTTAGTA400.00784478827.509452
GTGGTAT3250.025.3933411
CAGGTAT550.001220589625.008593
GACAGGT1101.1743396E-825.008591
ATCGTTT550.001220589625.0085937
GAGTTAA550.001220589625.008591
CATAGAA550.001222361325.00237349
AGGTATC550.001224135224.9961574
GTATCAA9700.024.9570271
AGAGTAC6200.024.3914111
TGGTATC3450.023.921262
CAGAGTA6450.023.44600710
TATCAAC10550.023.2070242
TCGTTTC600.002025678822.92454138
CAATCGT600.002025678822.92454135
AATCGTT600.002025678822.92454136
ACAGGTA752.8546175E-422.007562
AACGCAG11300.021.4126386
CATATCC650.00322414721.1611163
Produced by FastQC (version 0.11.5)