FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125432
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.1428784680016207No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.12510751427007585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.044.9400181
GTATCAA30750.036.767261
TGGTATC10850.034.863082
CAACGCA35200.033.445885
TATCAAC36200.032.991592
ATCAACG36500.032.487543
TCAACGC38100.031.1232344
GGTATCA17600.030.6701431
TACGCGC503.0952215E-629.75113558-59
AACGCAG40500.029.5937236
GTCTAAC905.1521285E-528.3356741
CGCAGAG41850.028.0272528
GTTATAC1106.494185E-627.0464883
GCATCGT1755.5479177E-1026.712938
ACGCAGA43800.026.6824367
AAGTACG2105.456968E-1226.3116971
GCAGAGT44900.026.124569
GTCGAGG5550.025.268998
CAGTACG1201.2762348E-524.7915129
CAATGCG951.4424586E-924.60510818-19
Produced by FastQC (version 0.11.5)