FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108006
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16690.15063095326198594No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.11660586675523417No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA250.007797832650.9972658
GTATCAA31500.038.3240551
TATCAAC34800.037.0090032
TGGTATC9450.036.8829882
GTGGTAT9750.034.8777961
ATCAACG37350.034.3684843
CAACGCA37500.034.1176645
TCAACGC38700.033.1695824
AACGCAG40750.031.7095436
CGCAGAG42750.029.723558
GGTATCA16550.029.5368021
ACGCAGA44750.028.6839087
GTCGAGG5600.027.3199658
GCAGAGT47850.026.5543279
TCGAGGT6100.024.3828379
AGTCGAG8200.023.3250777
GTGTACG1102.0135041E-423.1858061
ACGGGCG1302.3687675E-522.887525
AGCGTCA2951.8189894E-1221.612913
AGAGTAC24150.021.55681410-11
Produced by FastQC (version 0.11.5)