FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108006
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28380.25613579709857165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9300.033.7150732
GTGGTAT10350.030.8429011
GCACCGA400.007873827.49943712
GTATCAA32250.026.9646591
TATCAAC34850.024.702662
AGAGTAC21600.023.29813411
ATCAACG37150.022.9439323
ACGGATC600.00203572722.9161959
TCAACGC37750.022.6510754
CAACGCA37850.022.5175745
AACGCAG39450.021.7436966
CAGAGTA23600.021.44023910
GTATTAG1801.0913936E-1121.4039081
CCTTGCG650.003240911321.15245634
AATCCGT650.00324430621.1486386
GGTATCA16300.020.5972771
TGCTCCG700.004983012619.6380215
TGCCGAC2400.019.48052655
AGTACGG26000.019.14383713
CGCAGAG44800.019.0286278
Produced by FastQC (version 0.11.5)