FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907347
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.16531712784634764No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.16156993961516378No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.1006230251491436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8800.039.6115721
TGGTATC9350.039.093622
GTATCAA28150.037.90411
TCTAACG450.00166229537.7804032
TATCAAC32100.033.6316152
ATCAACG33350.032.5002983
CAACGCA34000.031.878975
TCAACGC34350.031.5541484
GTACTAG1950.030.5199831
ACGCCTA703.9397168E-430.3609226
AACGCAG36600.030.078886
GGTATCA15100.029.8414421
CGCAGAG39200.027.430248
ACGCAGA40150.027.0987667
GCAGAGT41850.025.6933169
AACCGTG850.001226253525.0003577
TACGGGT1601.8282117E-723.9092274
GAACCGT900.001709548823.6140526
CGGGACG450.001711302823.61144816-17
GTATAAC1452.0732004E-623.453781
Produced by FastQC (version 0.11.5)