FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907347
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.2134795177589169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT303.4928795E-545.8283278
GTGGTAT10600.038.9408951
TGGTATC10800.038.1944852
TGCGACG705.7051475E-627.50002922
GGTATCA16950.025.3083653
GTATCAA32750.025.0394921
ATGCGAC901.4464895E-624.44446821
GGTCGTC1502.3646862E-1123.83335752
AGAGTAC19000.023.01191311
GTACTAG1850.022.3120821
TATCAAC37050.021.8961092
CAGAGTA20700.021.3889110
CGACGAG903.9961003E-521.38773224
GACTCGT1055.531474E-620.9454737
TACTAGG1851.8189894E-1120.8108312
ATCAACG39000.020.5886293
CAACGCA39500.020.2528135
TCAACGC39750.020.1957114
CTAGTAC1802.6921043E-1019.8600373
GTCCTAT1401.5186197E-719.6558841
Produced by FastQC (version 0.11.5)