FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858613
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.11448696910016504No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.11169176334390465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6600.038.6392251
GCGTCAG1750.036.4312674
GTATCAA20200.036.401211
TATCAAC23100.034.771252
ATCAACG22950.033.887453
TGGTATC7300.033.765692
AGCGTCA1950.032.6909183
CAACGCA23850.032.6124765
TCAACGC23850.032.6124764
AACGCAG26300.029.8976526
CGTCAGA2150.029.6533585
CGCAGAG27550.028.232588
GGTATCA11850.026.9007241
GTCGAGG3650.026.7828038
ACGCAGA29550.026.753257
CTAGTAC1001.0559203E-425.4989173
GCAGAGT31850.024.9532999
GTACTAG900.001709977323.6128581
TCGAGGT4250.022.0003479
CAGAGTA16500.021.63670310-11
Produced by FastQC (version 0.11.5)