FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858613
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23050.26845621950750803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG401.8897672E-434.4101331
CGACGAG401.9021046E-434.3720524
TGCGACG401.9021046E-434.3720522
GTGGTAT6200.031.9681241
ACGCGCA350.004098444731.42953735
GTATACG653.1790441E-629.6456531
TCGTCTA752.945908E-729.33081654
ACCGTCC400.007873127.4992438
CGTCTAC858.786719E-725.88314855
GTACTAG759.6717085E-625.6928981
GTATCAA22650.025.6442851
TGGTATC7250.025.4190142
AGAGTAC15200.023.88092211
TATCAAC24550.023.3041782
GTCGAGC600.002035438522.9160378
CGAGTCC600.00203612822.91470127
CAATGCG600.00203612822.91470119
ATCAACG25400.022.4160233
CAGAGTA16250.021.99939310
CAACGCA26200.021.9351435
Produced by FastQC (version 0.11.5)