FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277824
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29890.23391327757187216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12300.037.3632771
TGGTATC11950.037.2769662
CGCGCAG350.004102403831.424510
CGTAACG952.4992914E-928.94475223
GTATCAA44300.027.581181
TTATACG400.00786490227.50612635
CCCGTTG507.0360693E-427.49751326
GTACGCA1156.657501E-1026.29991111
GCGACCG1057.2705006E-926.18708418
CGCCGCA1101.1894372E-825.00361441
TATCAAC49400.024.7143672
AGAGTAC31350.024.5572611
ACGGGTA901.4489087E-624.44127816
CGACCGT1151.913213E-823.90994619
ATACGAC701.7985351E-423.5757637
CAGAGTA33750.023.0562710
ATCGCCG1202.995148E-822.92087639
CGTGCGA600.002033943822.91997741
CCGTAAC1203.0013325E-822.91638822
CAGTACG1203.0051524E-822.9136989
Produced by FastQC (version 0.11.5)