FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106910
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34990.3161051937375216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT150.00578129755.0054337
CGACGAG1100.034.99712824
CGCGTTA401.8988844E-434.38305347
GTGGTAT10850.033.4610331
CGCGCAG350.00410031131.4274110
CGACGCT350.00410121831.4259933
GACGCCT350.00410121831.4259923
TGGTATC11800.030.7546232
CGATCGT453.7972844E-430.55304526
TGCGACG1350.028.51875322
AACGCTC705.6962963E-627.50644147
AACTACG400.0078692227.50271242
TCGTCTA904.7148205E-827.5027154
ATGCGAC1600.025.78146221
CAATGCG1500.025.6668819
CTAACCG550.0012267924.9990759
CGGTCGT550.0012267924.99907510
TCGCGTA550.001227113324.99794823
GCATCGT1650.024.9945568
GCGACGA1551.8189894E-1224.83666823
Produced by FastQC (version 0.11.5)