FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088664
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.11803458183608533No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.11472777643056077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8600.039.546681
GTATCAA30000.037.2694471
TGGTATC9600.037.1900522
TATCAAC33000.035.9342842
CAACGCA34800.033.8313455
ATCAACG35150.033.7363133
TCAACGC35400.033.3779954
GTACTAG2450.032.9690171
AACGCAG37600.031.6511086
TACCGAC703.9436412E-430.356447
GCGTCAG2600.029.4250974
GGTATCA16200.029.3914811
CGCAGAG40200.029.2841458
ACGCAGA41000.028.8164047
AGCGTCA2550.028.3352783
TATACCG755.9030775E-428.3352785
GCAGAGT44650.026.460759
AAGTACG1658.6402E-925.765261
CTAGTAC2800.024.2873823
TGCGACG853.290479E-722.49947722-23
Produced by FastQC (version 0.11.5)