FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927195
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.14538473568127525No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.14204131816931712No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG409.3125115E-442.5055272
GTGGTAT8600.042.0158121
TGGTATC9200.039.733432
AGCGTCA2150.037.5630263
GCGTCAG2200.036.709324
GTATCAA25150.034.4813651
GTCGAGG4500.033.0527278
TATCAAC29400.030.6502442
ATCAACG30100.029.7962343
TCAACGC30000.029.7538684
GGTATCA13950.029.5590061
CAACGCA30550.029.3573385
TCGAGGT4850.028.9166079
AGTCGAG5900.028.8110927
CGCCGTT300.00680590528.33396378-79
AACGCAG33200.027.1420846
CGCAGAG36250.024.618588
CAGCGTC3450.023.4088422
ACGCAGA38850.023.080397
CGTCAGA3500.023.074435
Produced by FastQC (version 0.11.5)