FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979417
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.13130260144555383No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.12272607071349588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG354.8298156E-448.564958
GTATCAA22650.038.8398481
ATCAACG26850.034.6601873
TATCAAC27000.034.4676252
CAACGCA27550.033.6252825
GTGGTAT7900.032.815431
TCAACGC28700.032.425994
AACGCAG29600.031.2965016
CGCAGAG30650.030.085718
ACGCAGA32200.028.6374847
TGGTATC9400.028.4802472
GTACCGC600.006809531228.3295566
TCGTACG600.006809531228.3295564
GTCGAGG4600.027.7136978
GGTATCA13050.027.3554671
GCGTCAG2350.027.1240444
GCAGAGT35900.025.9227289
AGTACCG1001.0567212E-425.49665
AGCGTCA2351.8189894E-1225.3157753
CTACTAG1502.790177E-622.6659581
Produced by FastQC (version 0.11.5)