FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971070
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.15652836561730873No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.14334702956532486No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.10164045846334456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9350.042.732452
GTGGTAT9950.040.5869871
AGCGTCA2200.038.641053
GCGTCAG2450.034.6998674
GGTATCA16350.033.7996251
GTATCAA28600.032.5511
TATCAAC33000.029.7536052
CAACGCA33900.028.9651785
TCAACGC34650.028.4609034
ATCAACG34250.028.4195023
AACGCAG36000.027.039396
CGCAGAG38000.025.3886188
ACGCAGA39050.024.814797
CAGCGTC3500.024.2886562
ATACCGA1102.0132454E-423.1858226
CTGTGCG1102.0160573E-423.1810469
GCAGCGT3950.022.6000041
GCAGAGT43200.022.4298089
CCGATAC950.002341198322.371133
CCCGTCG950.002342599722.3688268
Produced by FastQC (version 0.11.5)