FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971070
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20730.2134758565293954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10100.032.9650271
TGGTATC10150.032.511432
CCGATAC651.0886123E-425.3852333
CTTACGC600.002035014822.9172253
GGTATCA18200.022.2246741
GTATCAA31700.021.526921
GTCGACA956.0596227E-520.2626114
TCTTACG700.004976268419.6423242
GCCGATA700.004976268419.6423242
CTAGACA2005.2750693E-1119.2474944
TATCAAC35000.019.0923372
AGAGTAC17550.018.64621711
ATCAACG36050.018.460923
ATTCGGT750.00741003418.3337837
GCTCGTT750.00741003418.3337822
CGGCTAT750.00741223618.33283654
CTGTGCG750.00741223618.3328369
TCCGTGC909.819348E-418.331898
CAACGCA36300.018.1775785
AACGCAG36600.018.10376
Produced by FastQC (version 0.11.5)