FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993379
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24200.24361296141754557No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.18854837881614167No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.13771178976000098No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.1327791306238606No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12620.12704113938386052No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10368650837193055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13750.051.0048031
TGGTATC14350.049.760782
AAGTACG3950.043.0420271
ATACGGG2000.036.1284031
AGCGTCA1550.035.6485183
GTATCAA45500.033.7229581
TATCAAC56150.033.3067862
GTCGAGG11600.031.1420368
ATCAACG59550.031.0482653
GGTATCA25100.030.9889763
CGTAATC550.004445670630.9120042
CAACGCA59950.030.9125
GCGTAAT703.9406822E-430.3600041
TCAACGC61150.030.235884
CTAGTAC1551.3460522E-1030.1641313
TCGAGGT11800.029.5322199
AACGCAG63150.029.3455966
GTACTAG1554.447429E-927.4219381
AGTCGAG15550.027.3310137
ATACCGA1159.166082E-625.8726
Produced by FastQC (version 0.11.5)