FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993379
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21080.21220500936701905No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.12110181511789557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA257.853415E-444.002945
GTGGTAT14100.039.4255681
TGGTATC14500.038.1308862
TGCGACG300.00192582636.66539422
GTGACGT401.9006236E-434.37726645
TTTCGGC350.004100066631.42747517
GCGAGGA350.004100066631.42747537
GTACTAG901.3897079E-930.5775871
CGTCTAG400.00787009427.50181246
CTAGTAC1103.947207E-1027.5004273
GCCGATA400.00787396327.49904327
ATGCGAC400.00787396327.49904321
CATTGCG858.7979424E-725.8801529
GTCGTCT901.4465368E-624.44482653
GTATCAA50450.023.2923011
CCGCACT600.002036343822.9147174
GGTATCA27800.022.3722361
TCGTCTA1003.6139809E-622.00034154
AGGTCGT1003.6155598E-621.99923322
GCGCCAG1003.6171405E-621.99812733
Produced by FastQC (version 0.11.5)