FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775866
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30970.3991668664434322No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16520.21292336563272524No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9900.12759935349660895No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9270.11947939463773384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCT150.00578411154.997126
GTGGTAT11650.044.6315231
CGCCGGA257.856935E-443.9976814
GTACCGA257.8594306E-443.9948436
TGGTATC13250.039.0218432
GTATACG300.001921472536.6812861
GTGCGAA300.00192451736.6694640
AGTGCGA300.001925126436.667139
TATACCG300.001926345536.662375
CCGCCGG350.004099877631.42691613
TACATCG400.00786617827.5038746
CGTTGTT507.0320105E-427.4985528
CGACCGC400.00787360727.4985510
CCCGTTG606.317426E-527.4985526
CCGACCG400.00787360727.498559
CGCCGCG400.00787360727.498554
CCGTTGT507.0320105E-427.4985527
TGTACCG507.034661E-427.4967775
GTATCAA45850.026.4009251
ACGGGGC759.751087E-625.66531216
Produced by FastQC (version 0.11.5)