FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75214
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.2805328795171112No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.22469221155635918No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1520.20209003642938814No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.15954476560214856No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.14890844789533864No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1130108756348552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT200.00321177563.7500041
TACAGTC200.00321177563.7500043
ATACGGG200.00321177563.7500041
AAGTACG454.3617547E-756.6666681
GTTCATA401.4451391E-553.1250041
ATATACT250.007768117451.05
CCAACCT250.007768117451.04
CCTTGAG250.007768117451.02
AGTATCA354.7980732E-448.5714261
GTGGTAT1150.048.043481
TGGTATC1150.048.043482
GTATCAA4450.045.8426971
TATCAAC5600.044.0178572
ATCAACG6050.040.74383
TCAACGC6200.039.7580644
CAACGCA6250.039.4400025
AACGCAG6650.037.067676
ATATCAA1352.5465852E-1134.6296271
TATATTG500.002772525234.05
CCACGCC250.00278132434.070-71
Produced by FastQC (version 0.11.5)