FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75214
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4280.5690429973143298No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.18480602015582206No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.11832903448826017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAT150.00576351854.99800513
TATGCTG257.810976E-443.9984056
GTGGTAT1000.041.303431
TGGTATC1100.040.0251582
TCAATAG300.001908307336.689732
CTGCCGA300.001914557236.66533755
AATAGGT300.001914557236.6653374
GAATAAT400.007777862327.535621
GTATCAA4200.027.5356181
AAGTTTT400.00782859527.49900433
AATAATT400.00782859527.49900436
CGGGGAA705.6161116E-627.49900217
CTCAAGC550.001216180924.99909248
GCTGCCG550.001216180924.99909254
GGTATCA2150.024.3338031
TATCAAC4600.023.9280852
AGAGTAC3300.023.33248711
CAGAGTA3200.023.20228410
GTGTATA600.00200284422.9463481
CGCTGCC600.002018425422.91583653
Produced by FastQC (version 0.11.5)