FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57777
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2220.3842359416376759No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.12980943974245807No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA202.5948373E-454.99133320
TTAAACG257.8004523E-443.9930722
GTGGTAT950.043.4518621
TGGTATC1150.038.288022
CTGCTTA300.001912002336.6608918
GCTTAAA300.001912002336.6608920
ATACCAC300.001912002336.6608912
CTTAAAC350.00407087531.4236221
TAAACGC350.00407087531.4236223
GTATCAA3950.026.474471
CAGAGTA2750.024.0170310
AGAGTAC2750.023.99621811
TATCAAC4650.022.4890652
GGTATCA2150.021.7596171
AACGCAG5000.021.465226
CGACGTT903.8903006E-521.40406610
TCGACGT903.8903006E-521.4040669
CAACGCA4900.021.3416655
ATCAACG4950.021.1260933
AGTACGG3150.020.94908113
Produced by FastQC (version 0.11.5)