FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121724
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5430.44609115704380403No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.17005684992277612No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.11501429463376162No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA150.005764062555.0172754
GTCGTCT150.005764062555.0172753
TAGGTCG150.00577345154.9946621
CGGGCCG150.00577345154.9946617
CTTAACT202.6108712E-454.9720654
TGGCGAC257.816613E-444.01381345
AAGGTCC257.8324665E-443.99572827
CAATTAG257.8324665E-443.99572817
ACACTTC358.62702E-539.281923
AATCGCT300.001915912536.6781848
TCGTCAT300.001919786436.66310525
GTTCACC300.001919786436.6631051
TAATATC300.001923666636.648044
GTGGTAT1300.035.9580461
TGGTATC1300.033.828962
CGAGGGT601.7018574E-632.0802210
TCGAGGG601.7018574E-632.080229
CTGAACT350.00408731431.42551813
GTATCAA7850.031.1753141
GAGGGTT752.9112016E-729.33048211
Produced by FastQC (version 0.11.5)