FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72278
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.21721685713495115No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.1950801073632364No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.1480395140983425No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1040.143888873516146No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.10791665513710949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1101.09139364E-1038.6363682
GTCGAGG805.319671E-737.1875048
TCGAGGT805.319671E-737.1875049
GTATCAA3250.035.3076931
GGACTGG500.00277180734.06
GTGGTAT1254.311005E-1034.01
TATCAAC4100.033.170732
ATCAACG4750.028.6315783
AACGCAG4750.028.6315786
TAATACA600.006764081328.3333324
CAACGCA4800.028.3333325
GTTGTAG300.006786203528.33333230-31
GGTATCA1652.6921043E-1028.3333323
AGTCGAG1203.903042E-728.3333327
TCAACGC4900.027.7551044
CAAAGGT650.00999518826.1538454
AGAGTAC3200.025.8984410-11
CGCAGAG5550.024.5045058
TACGGGG2100.024.28571514-15
ACGCAGA5750.023.6521747
Produced by FastQC (version 0.11.5)