FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81874
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.19542223416469212No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.1868725114199868No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.14045973080587243No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1110.13557417495175514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC150.001026182284.998783
CGGTGGT200.00321297563.7490847
ACGGGTA250.007771002650.9992685
GTCGACG452.9030021E-547.2215468
AAGTACG801.0955773E-842.551381
GTGTACA409.2136546E-442.551381
TCGACGT505.4080105E-542.499399
TGGTATC951.1186785E-940.262582
GGTATCA2050.039.4378621
TACGGGT559.4850475E-538.635814
GTATCAA3450.037.0011981
GTGGTAT1104.636604E-934.8147621
TTATACT500.002774149233.999514
TATCAAC4150.032.7706152
CAACGCA4350.032.2409135
AACGCAG4500.031.166226
GTCGAGG550.00442446430.9086488
ATCAACG4400.030.9086483
TCAACGC4600.030.4886934
AACTCTG600.00676974128.3329265
Produced by FastQC (version 0.11.5)