FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46048
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.2736275191104934No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT640.13898540653231412No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1346421125781793No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.13029881862404447No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT480.10423905489923557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC150.0010203385.060941
TAAGTCA200.00319473163.7957044
TACAGAG200.00319473163.7957045
GTCGAGG454.3353793E-756.6456538
GGTACTG250.00772706551.036565
ATATCAA250.00772706551.036561
GGATTAT250.00772706551.036561
TCGAGGT452.8891025E-547.2047129
GTATCAA2600.044.1662561
GACAGGA409.193652E-442.5304681
AGGATGG409.193652E-442.5304685
GGTATCA1153.6379788E-1240.6813161
TATCAAC3150.040.5052072
ATGTTGG450.001640698437.804861
GTGGTAT450.001640698437.804861
ATCAACG3750.034.0243763
AACGCAG3750.032.890236
TCAACGC3750.032.890234
CAACGCA3800.032.4574625
AGTCGAG802.2486502E-531.8978527
Produced by FastQC (version 0.11.5)