FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46048
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1780.3865531619179986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT558.407005E-735.028421
TGGTATC558.479183E-734.9903032
CAGAGTA1200.032.07444810
AGAGTAC1300.029.60718211
GTATCAA2400.025.2288041
GTCGAGG1001.2703094E-724.716258
CTGACGC901.3742156E-624.49097355
TATCAAC2550.023.718922
AACGCAG2700.022.3768526
GAGTACG1757.2759576E-1221.99390612
ATCAACG2750.021.9939063
CAACGCA2750.021.975
TCAACGC2750.021.974
AGTCGAG1254.4488843E-821.977
CGAGGTT903.897683E-521.38296510
TCGAGGT903.897683E-521.3829659
AGTACGG1801.0913936E-1121.38296513
GGTATCA1107.991162E-619.994463
GTACGGG1953.274181E-1119.73812114
GCAGAGT3150.019.2010299
Produced by FastQC (version 0.11.5)