FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55826
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.2812309676494823No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.2525704868699173No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.23465768638268905No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.20957976570056963No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.16838032457994484No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT810.1450936839465482No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12001576326442875No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT650.11643320316698312No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10747680292336903No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.10568552287464623No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.10568552287464623No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT570.1021029627772006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG259.08313E-567.989961
ACAGCAC259.08313E-567.989968
CTGCACG200.003209303663.740599
GTGGTAT900.061.3798261
TGGTATC900.061.3798262
GTATCAA3800.040.2572171
TATCAAC4250.039.99412
GGTATCA1900.038.0207021
TACGGGT250.00278027433.9949814-15
CGGGGTA250.00278027433.9949816-17
ATCAACG5150.033.0048373
CAACGCA5350.032.5652855
TCAACGC5250.032.376174
ATATCAA550.004415381230.9045311
TCGAGGT1101.8112041E-730.9045319
AATACAG550.004415381230.9045315
AACGCAG5650.030.8361576
TGCTTAA300.006784564328.32915118-19
GTCGAGG1255.545844E-727.1959848
CAGAGTA3150.026.30564110-11
Produced by FastQC (version 0.11.5)