FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55826
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100.3761688102317916No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.2525704868699173No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14151112384910258No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1253896034105972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC651.9208528E-938.0489852
GTGGTAT703.965397E-935.33121
CGGGGAA956.730261E-1131.84730317
GCTGCCG350.004063285431.43370255
AATCTAG350.004063285431.43370243
GGTATCA1850.029.7079141
GTATCAA4100.028.150061
ACGGGGA1203.45608E-1127.50448816
AGAGTAC3350.024.63088211
TCGAGGT1700.024.2686659
TATCAAC4650.024.2295192
GGGGAAA1502.1827873E-1123.83722518
CAGAGTA3400.023.4597110
CGAGGTT1653.6379788E-1223.33714310
ATCAACG4950.022.7610653
TCAACGC4950.022.1860184
TACGGGG2250.022.0035915
GTCGAGG1901.8189894E-1221.7140718
GAGGTTT2050.021.46691711
CAACGCA5050.021.2030245
Produced by FastQC (version 0.11.5)