FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57734
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.4156995877645755No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.38105795545086085No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.34295215990577477No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.31523885405480306No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1490.25808016073717394No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.22690269165483076No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.21997436519208785No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.21997436519208785No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.19226105934111615No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.17147607995288738No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT870.15069110056465862No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1281740395607441No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.1281740395607441No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.11604946825094398No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.11258530501957252No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.1056569785568296No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10392489694114387No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATC200.003207981663.7500043
GTATAGA200.003207981663.7500041
TGGTATC1600.061.0937542
GTGGTAT1500.056.666671
AAGTACG508.9895366E-751.01
ATGATGG250.007758998351.03
AGTATCA508.9895366E-751.01
ATATCAA1000.051.01
TATAGAT250.007758998351.02
GATCTCA250.007758998351.06
TGATGGT250.007758998351.04
ATGGTAT409.24587E-442.5000041
GGTATCA3350.035.522391
CTGCTTA250.002778652834.018-19
TATCAAC7050.033.7588652
GTATCAA5550.033.6936951
ATCAACG7700.031.461043
TCAACGC7850.030.8598734
CAACGCA7900.030.1265815
TGTCTGA300.006780619728.33333664-65
Produced by FastQC (version 0.11.5)