FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57734
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3490.6044964838743202No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.5369453008625766No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.35334464959988915No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.20958187549797347No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12470987632937265No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.12124571309800117No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA150.005756628254.998272
GTCGACA150.005756628254.9982714
CCCGTTG150.005756628254.9982726
TCCCGTT150.005756628254.9982725
GTGGTAT1550.046.2076641
TGGTATC1650.043.331972
GCTGCTG400.00781354927.49913455
ACGGGGA1400.027.49913416
GTATCAA6550.027.3365941
TATCAAC7200.025.207542
TACGGGG2650.024.90487515
GGTATCA3350.024.6688421
GTCGAGG3500.024.3563758
AGAGTAC4750.023.73609411
CGAGGTT2900.023.7061510
TCGAGGT2900.023.706159
TCGAGGG701.772315E-423.5706869
CAGAGTA4800.023.48884410
AGTCGAG4000.023.3742647
AGGTTTT3000.022.91594512
Produced by FastQC (version 0.11.5)