FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80083
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC2410.3009377770563041No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT2250.28095850555049134No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC2100.26222793851379195No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA2050.2559844161682255No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA2020.25223830276088555No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT1660.20728494187280694No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC1520.18980307930522083No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT1430.17856473908320117No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA1270.15858546757738845No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG1230.15359064970093528No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG1210.1510932407627087No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.1460984228862555No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA1150.14360101394802893No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT1120.13985490054068905No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.13735749160246244No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG1050.13111396925689597No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG1030.1286165603186694No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC1010.12611915138044277No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA970.12112433350398961No Hit
GGAGAAGCAAGCAGGCGAATCGTAATGAGGCGTGCGCCGCCAATATGCAC830.10364247093640347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCA150.001026040684.999995
TAGGCAG150.001026040684.999996
AGCGTCA200.00321253463.753
GCGTCAG200.00321253463.754
GCCACAC250.00776993851.01
CGTCAGA250.00776993851.05
GGTATCA1600.047.81251
GTGGTAT551.7425355E-646.3636321
TGGTATC805.3292933E-737.18752
GTATCAA3200.034.531251
TATCAAC3600.034.236112
ATCAACG3700.033.310813
TCAACGC3700.033.310814
CAACGCA3750.032.8666655
AACGCAG3800.032.4342086
CGCAGAG4150.029.6987958
ACGCAGA4250.029.07
GACAGGA755.857374E-428.3333321
ATTGTAC300.00678836228.33333262-63
ATATCAA600.00676838928.3333321
Produced by FastQC (version 0.11.5)