FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80083
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT2220.27721239214315146No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT1930.2409999625388659No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1880.23475644019329947No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT1770.2210206910330532No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC1740.2172745776257133No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT1490.18605696589788095No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTC1430.17856473908320117No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTGCCTTTCCCATC1420.17731603461408788No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCACTTCTTCCACCC1420.17731603461408788No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCA1390.173569921206748No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAG1330.16607769439206824No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGGTTTGTCAGACA1090.13610878713334915No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCACT1080.13486008266423585No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGG1040.12986526478778265No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT970.12112433350398961No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTC910.11363210668930986No Hit
GTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCT910.11363210668930986No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCT880.10988599328196995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT508.0272E-944.0740931
TGGTATC604.0092345E-836.728412
CCAGCCC300.001910892536.682537
GTGTATA453.727379E-430.607011
GGTATCA1650.028.3810441
GAATGGC400.00783756327.49469232
GAATGTA651.0643622E-425.4273622
GTATCAA3150.025.3600921
GTATGTA550.001204715725.0420971
TATCAAC3400.022.6851962
GGAATGT752.8082595E-422.0370461
TTGCCAG650.00320576721.16298134
CAGTGCA955.9788385E-520.2592459
TACAGTG955.9788385E-520.2592457
TCAACGC3800.019.547914
AATGTAC856.562851E-419.4322933
TGTACAG856.590581E-419.4201475
ATCAACG3700.019.3447573
AGTGCAT1008.868895E-519.24628310
CAACGCA3750.019.0749025
Produced by FastQC (version 0.11.5)