FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112800
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.1675531914893617No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1440.1276595744680851No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10815602836879432No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC150.001027883384.9844748
TACCGGA200.003218275363.7383657
TAAGGGC250.00778376150.990694
TGTACCG250.00778376150.990695
TATACTT452.912955E-547.2136045
GTATCAA3200.043.8201261
TGGTATC1200.042.4922372
GTGGTAT1153.6379788E-1240.6447531
TATCAAC4250.038.992882
GGCTTAG450.001658181537.770887
CTGGATC450.001658181537.770889
CAACGCA4300.037.5512855
ATCAACG4400.036.6978453
TCAACGC4450.036.285514
AACGCAG4500.035.882346
CAGTACA601.5881412E-435.41024
CGCAGAG4600.035.1022878
ACGCAGA4700.034.355437
GTTTATA500.002780876633.9937932
GCAGAGT4900.032.9531679
Produced by FastQC (version 0.11.5)