FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112800
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3200.28368794326241137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCC150.005763500555.0159767
CTTAACG150.00577363554.9915714
GTGGTAT451.7809543E-742.7902071
AGGTCAC350.00407829431.4377046
GTGTAAT350.00407829431.4377041
TTGTGAG350.004087169731.42375652
GTATCAA3700.031.2252851
GACAGGT453.767634E-430.5644321
AGACAGT506.980492E-427.507996
ATTAATG400.00783241927.5079883
GTATAAA400.00783241927.5079881
GCTTTAA400.00783241927.5079881
TAATCTC400.00783241927.5079885
TATCAAC4300.026.8682692
AGAGTAC2800.025.54313511
ACAGTAC550.00121763425.0072638
ACGGGAA550.001220791424.99616816
GGTATCA1900.024.612411
CAGAGTA3000.023.84025810
CAACGCA4650.023.6627865
Produced by FastQC (version 0.11.5)