FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51412
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.20423247490858168No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT670.13031976970357115No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT600.11670427137633237No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT600.11670427137633237No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC530.1030887730490936No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT520.10114370185948807No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTC520.10114370185948807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC150.005710922655.100393
TGGTATC608.5310603E-1041.3252942
GTGGTAT651.8681021E-938.1464231
GGTATCA852.5585905E-829.1707943
GCTCCCC400.00780924327.49659518
GTATCAA1553.274181E-1123.1066131
GTACAGT600.001988106422.9584961
CAGAGTA1306.68806E-821.15122810
TCTGACG804.3669456E-420.62244655
AGTCGAC804.3669456E-420.62244616
AGAGTAC1359.964424E-820.3678511
TATCAAC1802.4374458E-1019.8973642
ATCAACG1905.020411E-1018.8501343
TCAACGC2001.0095391E-917.890194
CAACGCA2001.022272E-917.8727875
AGAGTCG1252.4114956E-517.5978214
AACGCAG2051.4206307E-917.4368676
AGTACGG1605.9484046E-717.18537113
TACGGGA1050.00270838915.71234115
GAGTCGA1406.370873E-515.71233915
Produced by FastQC (version 0.11.5)