FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38421
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.2472606126857708No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.1379453944457458No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC520.13534265115431665No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.12753442128002915No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT390.10150698836573749No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACA150.001021946984.999996
GTGGTAT555.456968E-1269.545451
CTTGTAC200.003199797863.7499923
GGCCACT200.003199797863.7499926
TGGTATC652.5465852E-1158.8461532
TTGTACT302.2277901E-456.6666644
TCTTAGA250.00773932950.9999962
CACTGCG250.00773932950.9999969
TATCAAC2450.041.6326522
GTATCAA2300.038.8043441
GGTATCA1251.0913936E-1137.3999983
ATCAACG3050.034.8360633
CAACGCA3100.034.2741935
TCAACGC3150.033.730164
AACGCAG3300.033.4848446
CGCAGAG3500.030.357148
ACGCAGA3550.029.9295777
GCAGAGT3750.028.3333349
CCACAAA300.006766700728.33333284-85
ACTGCGC300.006766700728.33333210-11
Produced by FastQC (version 0.11.5)