FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38421
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.2550688425600583No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT460.11972619140574166No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11191796153145415No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT390.10150698836573749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGA150.005741821654.998754
TTTTCCG150.005741821654.998754
CCTGGAA257.762592E-443.99895548
TGGTATC403.8416965E-641.249022
GTGGTAT458.54795E-636.71361
CGCTTTT300.001902787136.66580252
ACGGGGG401.8678914E-434.37418416
GCGCCTC350.004051391531.42782841
CACTGCG350.004051391531.4278289
CCTCGCT350.004051391531.42782844
CGCCTCG350.004051391531.42782842
GCTTCGC350.004051391531.42782848
TCGCTTT350.004051391531.42782851
GCCTCGC350.004051391531.42782843
CTCGCTT350.004051391531.42782845
CGCTTCG350.004051391531.42782847
AGAGTAC1600.029.21805611
GTATCAA2500.027.5352041
TCCTGGA400.00778120627.49934847
ACTTCGC400.00778120627.49934824