FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23387
Sequences flagged as poor quality0
Sequence length91
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3531.509385556078163No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.8851071107880446No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.7268995595843845No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.6328302048146406No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1090.4660708940864583No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.4660708940864583No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT950.40620857741480315No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT870.37200153931671437No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA830.3548980202676701No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.3164151024073203No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA730.3121392226450592No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.2950357035960149No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.2864839440714927No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.26938042502244836No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA560.23944926668662078No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG540.23089750716209861No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT530.22662162739983754No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.22234574763757642No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT520.22234574763757642No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA510.21806986787531535No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.21379398811305428No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.18813870953948775No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC430.18386282977722665No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC410.1753110702527045No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA410.1753110702527045No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1753110702527045No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA400.1710351904904434No Hit
ATATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA330.1411040321546158No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA330.1411040321546158No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA320.1368281523923547No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT300.12827639286783254No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA280.11972463334331039No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGG280.11972463334331039No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.11972463334331039No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA270.11544875358104931No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA270.11544875358104931No Hit
GTATCAACGCAGAGTACGGGCCGCTCGAGTTGTTAATTAAAGTATATAAT270.11544875358104931No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11117287381878821No Hit
ATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11117287381878821No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA260.11117287381878821No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.10689699405652714No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA240.10262111429426606No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAAC240.10262111429426606No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10262111429426606No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.10262111429426606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA150.001019028584.955031
AAGTACG402.1791493E-974.3356551
GAGGCAC250.002768985533.98201420-21
GGCACAG250.002768985533.98201422-23
AGGCACA250.002768985533.98201420-21
GTATCAA7900.032.2614061
AACGCAG8050.032.187936
TATCAAC8500.030.98362
CAGAGTA4800.030.08824210-11
TCGAGGT1302.2502718E-829.4075139
ACAGAGC300.00675736828.31834624-25
CGGGCAG600.00668920828.3183466
CGGGAGG300.00675736828.31834616-17
GCACAGA300.00675736828.31834622-23
CACAGAG300.00675736828.31834624-25
GTCGAGG1353.2478056E-828.3183448
ACGGGGA905.4892917E-728.3183445
CAACGCA9550.027.5770285
ATCAACG9650.027.2912523
AGAGTCG5000.027.1856125
Produced by FastQC (version 0.11.5)