FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23387
Sequences flagged as poor quality0
Sequence length61
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2170.9278659084106555No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.41903621670158636No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC570.24372514644888185No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA480.2052422285885321No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA380.16248343096592124No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC360.15393167144139908No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA350.149655791679138No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA350.149655791679138No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1411040321546158No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11117287381878821No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA260.11117287381878821No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10689699405652714No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA240.10262111429426606No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10262111429426606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC300.001887573436.66523716
GTGGTAT1600.029.2176071
TTTTTGC400.00771994627.4989241
TGGTATC1800.026.027062
AGAGTAC4100.023.47469311
CGAGGTT2350.023.40334310
CAGAGTA4000.023.37408810
GTCGAGG2600.023.2683228
GTATCAA6750.023.2213151
AGGTTTT2400.022.91577312
ATACCAC600.001977102822.91577312
TCGAGGT2400.022.9157739
TATCAAC7200.022.5823022
GAGGTTT2450.022.44810511
GGTATCA3800.022.4333361
ACGGGGT752.7582023E-421.99914216
AACGCAG7300.020.3416736
TCGACGT1651.6898412E-919.999229
GACGTTT1651.6898412E-919.9992211
CGACGTT1651.6898412E-919.9992210
Produced by FastQC (version 0.11.5)