FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133346
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2830.21222983816537427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA202.60109E-455.0176548
TCGACAT202.60109E-455.0176549
AAACCGA300.001916498136.6784366
CAGAGCC300.001916498136.6784367
TAAGACA601.6979047E-632.093634
TTGGAAC350.004087839331.4268533
GTGGTAT2300.029.9008981
CACCTTC400.00785066827.49849712
TGGTATC2650.026.979662
ACGGGGG1309.640644E-1125.38322816
GTATGTA550.001218581325.0080261
GTATCAA6600.024.1744251
CGGGGGA600.00202679422.91541517
CAGAGTA4500.022.00706110
TATCAAC7450.021.7773322
AGAGTAC4550.021.75705111
AACGCAG7700.021.0781946
GGTATCA3550.020.1397443
CAACGCA8000.019.9439015
ATCAACG8150.019.9068873
Produced by FastQC (version 0.11.5)