FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166472
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3390.20363784900764093No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.1435676870584843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGA150.005773703655.00300611
ATTCCGT150.005773703655.00300626
AGGGCGG300.001920383736.6686723
GTGGTAT2700.035.671541
TGGTATC2850.033.7636262
ACGGGAT350.0040885631.43028616
CGGGATT350.0040885631.43028617
TCCGTTC350.0040885631.43028628
GTATTGA400.00782900327.5180451
GATACCA400.00785202127.50150311
CGTTCCA507.004259E-427.50150330
AGGCCGA507.004259E-427.50150323
CCGAGGC507.004259E-427.50150326
AGCTCAT400.00785202127.50150310
CCGTTCC400.00785202127.50150329
GTATCAA9700.025.5322071
AGAGTAC6750.024.44578211
GGTATCA5000.024.215881
TATCAAC10650.024.008182
CAGAGTA6850.023.68742610
Produced by FastQC (version 0.11.5)