FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265799
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.17664731920312782No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.17151222271466482No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13810.10910105000872966No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.1032549401603256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1650.074.692844
AGCGTCA1850.066.6179353
CGTCAGA2000.061.6215935
GGGTACC250.007799356250.9951678
GTGGTAT11450.048.996791
TGGTATC12250.047.8749052
GGTATCA23800.039.6437951
GTATCAA48200.039.1503031
TATCAAC59900.036.963732
CTTGCTC1151.8189894E-1036.9544753
TACACCG751.4843659E-533.998125
CAACGCA66800.033.0820055
CAGCGTC3750.032.864852
GTCTTGC1306.7848305E-1032.6930851
ATAGCGG652.5543274E-432.6905026
AGATGTG3900.031.599579
TCAACGC71100.031.3801214
AAGTACG5050.031.139351
ATATCGC1101.8632636E-730.9073816
ATACCGT550.00444938130.9073817
Produced by FastQC (version 0.11.5)